Narrative Essay -- essays research papers

Narrative Essay problems with format It was a normal Friday in March except for the fact that I got my license. So my friends and I thought about what we were going to do now that I have my license. Well, we cruised around town thinking we were hot stuff and that we were better that most kids in school since I had my license before many of my friends. We decided that we'd go off-roading since I had the huge truck. It was a hunter green 1979 Ford F-250 Custom. It had huge 33-inch tires, a loud crackling exhaust, and a unique 2-inch thick oak plank flatbed. It really was a beast. So we roared around the backroads hitting bumps and flying into fields just causing a little mischief not doing too much harm. After a week of horsing around in the truck, we became quite bored. So we decided to do something else. We sat around pondering what to do, nothing really came to mind. So my friends and I decided to go to a girl in our class's birthday party at the local recreation center. So on the way to the party we contemplated what we'd do afterward. My friend Chris brought to our attention the he just bought a brand new paintball gun. So with that in mind, we decided that we'd cruise around after the party and shoot houses with it. So we went to the party. We danced, talked with friends, flirted around with the girls, and had an all-around good time. So at the party we discussed who and where we'd go shoot the paintballs. We unanimously chose to shoot a girl's house that we really disliked. Till this day, neither of us knows why we dislike her. But anyway, Shannon Everett was her name and her house was the target. So as the party came to a close, Chris, Sise, and I sprinted to the truck excited about the attack on Shannon's house. We speeded to Chris's house so he and Sise could change and to get the Trracer, the beautiful new gun. While waiting for them, I could only feel the adrenaline pump in my body. As Sise and Chris pounced down the stairs, Cathy, Chris's mom asked why we were all dressed in black at 10 :30 at night. She asked what kind of mischief we were going to cause tonight. We just snickered and said we were going up to my house for a nice friendly game of Night Capture the Flag. Little did she know that what we were doing was nothing of the sort. So Sise and I meandered to the truck while Chris scurried off behind the house. A couple seconds later we sa... ...s wrong what I did. He then informed me that he had the State Police investigation called off. I was like, you had an investigation on us, damn. So afterwards, I hung up and called Sise and told him the story on what to do. He too agreed to call right away and apologize since I mentioned the State Police investigation. After that, I called Chris to see what was up. Turned out that our mischief and lying cost him a month of groundation, no phone privileges no paintball gun for 3 months, and no driving with me school for the rest of the year. Even though he got into a lot of trouble, I was scot free, and so was Sise. My parents didn't find out till my senior year when were shooting the word about paintball gun crimes in our area. Chris and I were there and stated that we made the precedent of the Lehighton Paintball Militia. We then explained what and when we did it. Still the troubling question of how Mrs. Everett found out was on all our minds. Turned out that my cousin Derek ove rheard us at the party talking about our little attack and decided to rat us out. Till this day, we still mention about the incident and bust on Derek for ratting us out and we know never to do this again

Cloning is Not What it Used to Be :: Cloning Argumentative Persuasive Argument

Cloning is Not What it Used to Be      Ã‚  Ã‚  Ã‚   On Sunday, February 23, 1997, Scottish researchers broke one of nature's greatest laws by cloning a lamb from a single cell of an adult ewe.   This breakthrough opens the door to the possibility for the cloning of other mammals including humans.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   This remarkable achievement is being looked at as a great advancement in animal agriculture.   But this achievement could lead to ethical questions of standard.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Researchers lead by Ian Wilmut of the Roslin Institute in Midlothian, Scotland, showed that a fully differentiated cell from the mammary tissue of an ewe could be manipulated in such a way as to produce a genetically identical copy of the animal that the DNA was acquired.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Scientist long believed that once a cell became differentiated, that most of its approximately 100,000 genes shut off.   Only a few genes remained active to allow the cell to perform its specific function of life.   All efforts to reactivate the shut-off genes have failed.   English researchers have came the closest by teasing frog body cells to develop into tadpoles.   The tadpoles, however, never matured into frogs.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The Scottish researchers have failed many times with sheep cells before their success, but the task was perfected and accomplished.   Now this accomplishment has made it possible for the cloning of almost any mammal, including humans.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   To the average person, exactly how the technique works is unclear. Scientist predicted that by making cells dormant and bringing them close to death, something happens to break the chemical locks (barriers) that keep most of the genes inactive. The mammary cell is inserted into an unfertilized sheep egg cell that has already had all of its own genetic material removed.   By fusing the cells together tricks the egg into thinking that it has become fertilized.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   After being fused together, researchers believe that the chemical machinery inside the egg cell goes to work to reprogram the mammary cell genes into starting over again, as if they were brought together as sperm and egg. The cell divides, produces an embryo, fetus and a newborn that is identical to the animal from which it was cloned.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Although the United States government prohibits government funds being spent on human cloning research, and ethicists decry it, nevertheless, human cloning could be achieved, Neal First said.   First is a professor of animal biotechnology and reproductive biology at the University of Wisconsin.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Overall, there is no apparent reason to clone humans. A duplicate body does not mean a duplicated mind.

The Predatory Organism: Bdellovibrio

Identifying the genes and the proteins to be expressed by this organism during pore formation in the host cell will be of significance to bacteriology. The possible use of this information can be seen in the study done by Kadouri and O’toole. It was established in this report that B. bacteriovorus can be used as a control agent against certain biofilm communities. It was observed that biofilm populations of Escherichia coli and Pseudomonas fluorescens were significantly reduced when exposed to B. bacteriovorus.Since biofilm formation is unnecessary and damaging in some instances, inhibition of its formation can be done through natural elimination of the biofilm organisms, and that is through the inoculation of B. bacteriovorus. Another possible application of this predatory organism is through its use as an anti-microbial therapeutic agent. It had been suggested by Hobley et al. that the introduction of this organism to a microbial-infected wound can lead to the reduction of p athogenic organisms in the site of inoculation.In this way, the use of antibiotics can be avoided and possible resistance of disease-causing organisms to the applied drug can be avoided. If the genes responsible for pore formation in the host cell will be identified, the organism can be manipulated to increase the genes’ expression. These can then be enhanced and controlled in accordance to the desired amount and time of expression. II. Aims This paper aims to propose a method on how to determine the necessary and significant enzymes involved in pore formation of the host cell by Bdellovibrio bacteriovorus H100.The specific aims are as follows: 1. Identify the genes of B. bacteriovorus H100 specifically expressed during pore formation in the host cell during the predatory life cycle phase of the organism using time course Microarray analysis. 2. Identify specific hydrolytic enzymes expressed by the identified genes of B. bacteriovorus H100 involved in pore formation on host c ell membrane. III. Background The discovery of Bdellovibrio by Stolp and Starr in 1962 opened the scientific world to the dawn of a new organism- a bacterium which preys on its own kind (12).This bacterium is a curved rod, Gram negative organism classified under the group of delta-proteobacteria. It is a motile bacterium; monotrichously flagellated; obligately aerobic; small in size (0. 3 ? m in width and 1-2 ? m in length); and is obligately predatory. Its genome consists of 3. 85 mega base pairs. Most of these genes encode for enzymes involved in hydrolysis and flagella involved in host sensing (3). The best known species of Bdellovibrio is B. bacteriovorus, observed to prey on other Gram negative organisms such as Salmonella, Escherichia coli, Sphaerotilus natans and Pseudomonas fluorescens (1).The distribution of Bdellovibrio bacteriovorus is observed in diverse environments; therefore, it is considered to be an ubiquitous bacterium. It was associated in dry environment such as the soil; in wet locations such as brackish water, sewage, fresh water, pooled reservoirs and sea water; and in unique microbial niches such as biofilms (3). The life cycle of this bacterium consists of two stages. The first stage is the so-called free-swimming attack phase and the second stage is known as the intraperiplasmic replication phase. Figure 2 shows a graphical representation of these two stages.The life cycle of B. bacteriovorus takes about three hours to complete. For the initiation of the attack, the monotrichous flagellum is an important facet to consider. Movement is essential in finding the suitable host in the environment. The predatory organism moves towards a region with a high prey concentration. This process is meditated by chemotaxis. It can be seen from the illustration that a critical stage in the predation of B. bacteriovorus is the ability of the organism to penetrate its host bacterium. Upon contact with another Gram negative, B.bacteriovorus then forms a pore in the cell membrane of its host. Initial entrance of B. bacteriovorus is followed by the organism’s penetration of the periplasm. Bdellovibrio bacteriovorus then occupies the periplasmic space of the host cell (3). Without this phase, the other steps in the whole life cycle of the organism cannot be ensured to occur. Figure 3 and 4 shows an electronmicrograph of B. bacteriovorus attaching to a host cell. A specific strain of the bacterium, B. bacteriovorus HD100, was studied by Rendulic, et al. This strain of Bdellovibrio was found to have an unusually large genome.Though this bacterium preys on other Gram negative organisms, its genetic make-up did not comprise of any gene from its host. Furthermore, it was elucidated that the genes present in B. bacteriovorus HD100 are made up of gene families coding for enzymes such as hydrolases and transporters, important in the penetration and killing of the host. These genes also code for enzymes needed for uptake of complex mol ecules (6). One hurdle in studying the molecular characteristics of this organism is its host dependent nature. Without a suitable host, growth cannot be ensured, thus, elucidation of its genetic make-up may be difficult to achieve.Further studies using this bacterium revealed that Bdellovibrio can generate mutant cells that do not require host cells for growth and are therefore known as host independent (HI) strains. Despite this, they were able to retain the ability to grow on prey and hence are termed as facultative predators. For gene manipulation techniques, HI strains are usually used (8). Despite the fact that the complete genome of the organism was already sequenced, the specific genes coding for the needed enzymes to form pores in the host cell were still unidentified.With this lack of information, this study is formulated and designed. IV. Research Design and Methodology Culturing of B. bacteriovorus HD100 on prey dependent and prey independent set-ups: Predatory (HD) cult ures of B. bacteriovorus HD100 will be grown on E. coli in Ca2_-HEPES buffer at 30Â °C, with shaking at 200 rpm (8). Escherichia coli ML35 and E. coli W7-M5 (10) will be used as the prey throughout the experiments. Escherichia coli ML35 will be cultured in nutrient broth (Difco Laboratories), and E. coli W7-M5, a lysine and DAP auxotroph, will be cultured in nutrient broth supplemented with 0.2 mM lysine and 0. 1 mM DAP at 37Â °C with shaking at 200 rpm. Prey-independent HI strains will be plated on rich peptone-yeast extract (PY) medium (8). Synchronous cultures: Synchronous cultures will be used for performing various experiments as described below. Briefly, fresh bdellovibrios will be added to prey cells in HM buffer (3 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-1 mM CaCl. LQ. One mM of MgCl2 will be adjusted to pH 7. 6 using NaOH (10). The organisms will be grown until a final concentration of 1010 bdellovibrios per ml and 5 x 109 E.coli per ml is reached. For proper aeration, volumes will be kept to ? 20% of the flask’s volume and incubated at 30Â °C with shaking at 400 rpm. Synchronous cultures will be examined at intervals for attachment and penetration with a Nikon model L-Ke microscope (Nippon Kogaku Inc. ) equipped with phase-contrast optics and a Nikon model AF camera. Time course Microarray analysis. Time course Microarray analysis will be performed to identify the genes to be expressed during the entry phase, specifically during pore formation on the host cell membrane of B.bacterovorus H100. Microarray slides of B. bacteriovorus H100 will be ordered from Advanced Throughput, Inc Services. Total cellular RNA will be extracted from B. bacteriovorus H100 cells at entry phase using the RNeasy mid kit (Qiagen). The RNA of the organism will also be extracted during the other stages of infection. This will serve as a reference for comparison of the genes expressed and not expressed at the desired stage. Complementary DNA sy nthesis, fragmentation, labeling, hybridization, staining and washing will be performed according to the Affymetrix B.bacteriovorus H100 GeneChip array expression analysis protocol (Affymetrix). Briefly, cDNA will be synthesized from RNA using Superscript II (Invitrogen) according to the manufacturer’s instructions. RNA will be removed by alkaline treatment and subsequent neutralization. Complementary DNA will be purified with QIAquick PCR purification columns (Qiagen). Purified cDNA will be fragmented by DNase I (Amersham) at 37Â °C for 10 min followed by end labeling with biotinddUTP, using an Enzo BioArray terminal labeling kit (Affymetrix), at 37Â °C for 60 min.Hybridization will be performed in an Affymetrix GeneChip hybridization Oven 640. Washing and staining will be performed using an Affymetrix Fluidics Station 400. Arrays will be scanned with an Agilent GeneArray Scanner G2500A. GeneChip scans will be initially analyzed using the Affymetrix Microarray Suite 5. 1 s oftware, from which PivotData tables will be exported. Raw data from the PivotData Tables will be analyzed in GeneSpring software version 6 (Silicon Genetics), using the parameters suggested by Silicon Genetics for analysis of Affymetrix Microarrays. Real-time PCR:Real-time PCR using the Applied Biosystems 7500 Real-time PCR system will be performed to confirm microarray results. RNA will be extracted from B. bacteriovorus H100 at initial phases of predatory life cycle up to entry phase as described above. RNA will be reverse transcribed into cDNA and simultaneously labelled using the iScript One-step RT-PCR kit with SYBR Green (Biorad). RT-PCR reactions will also be performed to amplify cDNA of housekeeping genes (identified from micro array studies) for normalization of fluorescence values. Identifying the specific hydrolytic enzymes of B.bacteriovorus which are involved in pore formation on host cell membrane. Many experiments showed that B. bacteriovorus H100 releases hydrolytic enzymes during predatory life cycle. According to Thomashow and Ritterberg, glycanases and lipopolysaccharideases are required for pore formation in the prey’s peptidoglycan and LPS layers respectively. The glycanase and/or peptidase could be responsible for weakening the peptidoglycan layer of the prey and thereby responsible for permitting conversion of the substrate cell to a spherical shape (10).Tudor et al. proposed another model for penetration. According to them peptidase is responsible for pore formation but not glycanase (11). Specific enzymes involved in pore formation are not known. The genes identified from the time course micro array technique will be mutated as described previously using suicide vector pSSK10. Resulting mutants will be complemented by using vector pMMB206 (8). Mutants will be analysed for the specific enzymes (using 2D-gel electrophoresis) and their actions on host cell i.e, as a glycanase, LPSase or peptidase will be observed by radio labellin g experiments (10). Wild-type B. bacteriovorus H100 and complemented strains will be used as controls. Radio labeling experiments: Escherichia. coli W7-M5, auxotroph for lysine and DAP and cannot metabolize glucosamine, will be radiolabelled as described previously (9,10). Peptide portion of E. coli W7-M5 peptidoglycan will be labelled with [3H] DAP and the lipopolysaccharides and glycan portions of the peptidoglycan will be labeled with [3H]glucosamine.Various mutants and wild-type strains will be tested for predation using this radiolabelled strain. Solubilisation of glucosamine and DAP from labelled prey peptidoglycan will be measured as described previously (11). Briefly, samples taken at intervals will be precipitated with an equal volume of cold 10% trichloroacetic acid for 30 min followed by centrifugation. Resulting supernatants will be assayed for soluble radioactivity in a scintillation counter (Rackbeta II). Two-dimensional gel electrophoresis: The hydrolytic enzymes rele ased by B.bacteriovorus H100 during its predatory life cycle will be analyzed by performing two-dimensional gel electrophoresis. Sample preparation for 2D-gel electrophoresis: Escherichia coli ML35 cells will be challenged with B. bacteriovorus H100 wild-type as well as the mutant strain. Culture fluid will be drawn from synchronous cultures during attachment and entry phases of B. bacteriovorus H100. Culture fluid will be centrifuged to discard any cell debris. Proteins in the supernatant will be precipitated using cold acetone. The precipitated proteins will be separated by centrifugation.The precipitated pellet will be air dried and will be dissolved in rehydration solution (8M urea, 2% CHAPS {3-[3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate}, 18 mM DTT, 0. 5% IPG buffer pH range 4-7; Amersham Biosciences), plus a trace of bromophenol blue. Sample protein concentrations will be determined using the BCA protein assay (Pierce). Resulting protein pellet will be subjected to 2D-gel electrophoresis. 2D-gel electrophoresis: Two-dimensional (2-D) gel electrophoresis will be performed according to the method of O’Farrell.Proteins present in the pellet will be resolved on two-dimensional gels using the products and protocols of Amersham Pharmacia Biotech. In the first-dimension, proteins will be resolved by isoelectric focusing on a precast Immobiline DryStrip with a linear pH gradient. This will be followed by electrophoresis using sodium dodecyl sulfate polyacrylamide gel on 12. 5% acrylamide gel. For analytical 2-D gel electrophoresis, 100 ? g of sample protein will be applied to the gels and the proteins will be stained with Pharmacia Biotech silver stain kit.For preparative two-dimensional protein gel electrophoresis, 500 ? g of the sample protein will be loaded on the gels and proteins will be visualized using Coomassie blue R-350 (Phast Gel BlueR; Amersham Pharmacia Biotech). Spot analysis will be carried out using PDQuest Image Analysis softw are (BioRad). Spots absent from the mutant sample gel will be manually excised from the wild-type B. bacteriovorus H100 sample gel for identification. Mass spectrometry and protein identification: Excised protein spots of interest will be destained, reduced, carboxymethylated, and digested with trypsin in situ.This will be done overnight with a temperature of 37Â °C as described (8). Gel digests will be centrifuged, and an aliquot of the supernatant will be taken for analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Lists of peptide masses will be generated and searched against the NCBInr database using the Mascot protein identification system (Matrix Science; http://www. matrixscience. com). Sequences of proteins identified by Mascot will be analyzed for a predicted signal sequence using SignalP 3. 0 (http://www. cbs. dtu.dk/services/SignalP/). V. Conclusion The identification of genes responsible for the expression of hydr olytic enzymes of Bdellovibrio bacteriovorus is of significant importance. Since the organism preys on other bacteria, its special attributes must be thoroughly studied and appreciated for possible useful applications. As mentioned earlier, this predatory organism can be used as a control agent against biofilm, as well as pathogenic organisms. Through the use of natural agents to control destructive and disease-causing bacteria, the use of chemicals and antibiotics can be lessened or avoided.Since the rapid evolution of organisms towards resistance and tolerance to anti-microbial substances is becoming more widespread, an alternative approach to these problems can be useful in the world of bacteriology. Thus, the identification of these genes and hydrolytic enzymes is of utmost importance. The elucidation of this information can be used as a primary defence against harmful microbes if this knowledge will be taken advantage. A more effective predatory role can be performed by Bdellov ibrio bacteriovorus if the genes and the hydrolytic enzymes of the organism will be expressed efficiently and constitutively.

The Renaissance era of literature

The Renaissance era of literature The Renaissance era embraces the period between 14th and 16th centuries. The term Renaissance itself meaner the rebirth what in some respect is referred to the rebirth from the obscurity of middle Ages and is originated from a French word. This period has influenced all of branches of human life including religion, philosophy, politics, music, science and literature. Taking into consideration the Renaissance literature it is to be mentioned that there are generally three periods of its development.These are Early Renaissance (14th century), High Renaissance (1 5th 16th century) and Late Renaissance (16th century). The Renaissance era in literature begun from the well-known Dante Aligner's The Divine Comedy. The more important writers of the Renaissance were William Shakespeare, Thomas More, Florentine Vacation, Christopher Marlowe, Ben Johnson, Miguel De Cervantes, Francesco Patriarch and Niccole Machiavelli. In theirs works the authors referred to t he ideals reflected in ancient civilizations like Roman or Greek. They incorporated the classical style into their own works.Religious works, lyric poetry, and drama were here major types of literature in the Renaissance era. This period is characterized by the emergence of new genre of short story. During the Renaissance great changes in world literature have occurred. The first and the most considerable one was the break with the Latin language in the literature. The writers started to write in national languages. The introduction of movable-type printing press in the 1 5th century stimulated great development of literature as well as eliminated the use of manuscripts.Comparing with previous era, books became cheaper and people in order to read a book did not have to know Latin anymore; thus, the books became more affordable in the Renaissance. The Renaissance generally and its literature particularly not only gave the brilliant group of outstanding authors to the world, but actua lly altered the course of the history as well as the literature. References Britton , J. (2006). The Renaissance: A very short introduction. New York, NY: Oxford University Press. Lewis C. (1980). Studies in Medieval and Renaissance Literature. United Kingdom, I-J: Cambridge University Press. (Lewis, 1980)

Act of kindness Essay

An Act of Kindness that I did for someone would be for my parents. What I did as kindness would be that I help my mom do chores at home without them telling me to help. I will see that they where always working hard and they will come home preatty late. So I kind of took care of home and my two brothers. and sometimes my dad too when he felt really bad, what I mean by bad would be that he had lukimia cancer. My mother work as a babysitter she will go to work mostly of the time at 6am and come back at 10pm and on weekends she would clean houses so she never had time to do chores at home or make dinner. My father worked in construction he was a hard worker too he will go to work a little later than my mom and come home earlier than her too. he was the one the took us to school when he could. Or one of my aunts would take us to school when he couldn’t. My dad also was battleing lukimia cancer for 10 years, he past away at age 41 september-29-2009. The Act Of Kindness that i did would be, first of all I would make breakfast for my brothers and dad so they could eat and so my dad can take his medication so ill help around with breakfast sometimes I will wake up later because i was tired I had to go to school and I was also young mother.By age 14 I had a baby too. And well my mom we didn’t really see in breackfast time because she would wake up eairlier she would even eat breackfast in the mornings. Sencondly I will come from school start cleaning the house wash dishes wash clothes clean the bathroom, and fix all the rooms my parents and brothers room vacume and mop . and if there was clothes to fold ill fold and put it away too. Then finally make dinner. so by the time by dad got home from work there will be dinner my dad would go to work sometimes right after he got out of his kimotheraphy he will go to work when he wasn’t soppost to. I will help my mom do all these chores because she worked alot barely had time to do it and ill see that she will come home tired. Act of Kindness Essay Today i will be telling you about one of my act of kindness, but before i do that i will be giving you hints as to what your about to read. It happen at a store that was one city over from me and was one of the best places to be be around because of the places near it. I was really young and it happen in the toy aisle because when your little and a boy it’s your favorite place. There was a young boy who was crying out for his mom. I was a worried alot when i was little and so i had my older sister help me look for the kids mother so he could smile. Those are my hints to what your about to read in my essay about my act of kindness. My act of kindness takes place at Kmart in Simi Valley, Califronia. I lived one city over from Simi and it was the only major shopping store close to us that was like a Walmart. I loved going to Kmart with my mom and sister because i would most likey get a toy for going with them. Once before when i went to Kmart with my mom i got lost from her and i t was the scariest thing a young boy can feel being lost from his mother. When i got lost there was someone there to help me get back to my mom, so i always felt that i should help someone else who was lost from someone they love. MY act of kindness happened in Kmart but to be more precise it happened in the toy aisle in Kmart. When your a young boy the toy aisle is like a candy shop and every toy is like a different flavor. I was six years old with my mom and sister and i would always go to the toys to look and see what i wanted for chrismas because chrismas was like a month and a half away. I saw a little boy in the toy aisle with me he had his hands on his face and i didnt know what he was doing. The little boy was three years old and his hands were over his face because he was crying and i did’nt know why he was crying i was thinking it was for a toy and he mom wouldnt let him get it. Then i hear him starting to cry mama mama where are you mama. I started to worry for the three year old boy even though i was only six years old i worried and to this day i still worry about stuff. When i went up to my mom i told her i think that boy is lost from his mom. I got to the point where i didnt want him to be sad anymore because i knew when i got lost from my mom someone helped me. I ask my sister if she would help me with looking from his mom, my sister was 12 so she was older than me and could do alot more than i could. We started to look around the toy aisle because that where the three year old boy was so we thought his mother would be close by. Then we started to look other places we went to the food  aisle and the young kids clothing aisle. We looked everywhere we were thinking his mom left Kmart and left her son in the toy aisle. We went to the cash register have an employee page that a little boy lost his mom, but right when we got there we heard the mom yell the three year old boy’s name and he yelled mommy and she thanked us for helping her son look for his mom and i was happy knowing he got back with his mom. That is one of my act of kindness but to me this is one of the biggest act of kindness i have done because i was six when i did this act of kindness. To some people it might not seem like a big act of kindness but even the littlest acts of kindness can help someone in the long run. So if i could say that someones act of kindness to me made me in turn help someone else. I hope what you get from this story is that you can help someone no matter the age or the place. These are my words of wisdom for someone who was helped and who in return helped someone else.

A Learning Experience: Lost in Translation

Ralph Waldo Emerson, a well-known American literary icon, once said that â€Å"No man should travel until he has learned the language of the country he visits. Otherwise he voluntarily makes himself a great baby – so helpless and so ridiculous.† I came upon this quote while I was looking for some passages or anecdotes that might make for a â€Å"first paragraph with a good hook.†Emerson’s take on the issue of language learning was somehow brutal and unconstructive, instantly attracting my attention since I am an international student acknowledging some limitations in my knowledge and skills in the use of the English language.Although Emerson was, and is still, highly-revered for his ingenuity in literature, I would have to express my disagreement about what he said about the helplessness and ridiculousness of people who visit countries without obtaining knowledge and practical skills on each country’s native language.My experiences in this country h as taught me that being at a disadvantage in terms of one’s lack of knowledge and skills in language and communication do not bring down a person to helplessness and nonsensicality. For I have discovered that being â€Å"lost in translation† is not a hopeless situation but an opportunity to sensibly and realistically learn the language.When arrived here one year ago, my limitations pertaining to the English language has made my life adjustments worse since it was difficult to openly communicate and relate to other people. Being in an unfamiliar place was stressful enough, having to deal with not being able to conveniently visit places, dealing with the changes in the weather, learning the culture, and such.Not being able to communicate well enough made it much worse, since it became a barrier in smoothing the progress of adjustment and adaptation. At first, I was deeply uncomfortable with having to talk to other people who are native speakers of English for fear of bei ng criticized or made of as a joke. However, my everyday experiences has taught me that gradual openness to socialization, interaction, and communication is a opportunity for me to learn not only the language, but the cultural practices and traditions that are unfamiliar to me.I found out how talking to other people often about anything helped in developing my knowledge and skills of the English language. But perhaps the most important skill that I have learned is how to use theEnglish language in practical, everyday conversations in order to avoid or prevent confusion that is rooted from misunderstandings or miscommunications. There was this one time when I was watching the evening news with a friend of mine. The news show televised a case wherein two individuals claiming to be insurance handlers fooled other people into subscribing for insurance plans.They ran away after obtaining the initial payments made by their clients. I remember my friend telling me initially how it was a â €Å"rip off.† I did not understand what he meant by the situation being a rip-off since the act of ripping to me, as I understood it, is the act of tearing or cutting a thing or an object. Out of pure curiosity, I asked him what he meant and he explained to me how the word â€Å"rip-off† means a form of cheating or conning.I especially value learning practical English language through my daily encounters with other people because it has greatly helped in my being able to adjust to my life here in a foreign country.However, I am not disregarding the fact that learning the formal grammar rules of the English language is all too important, my learning of practical English language for everyday use has contributed to my getting used to living in this country as I feel that I understand people more when I talk to them leaving out confusion or awkwardness in misunderstandings or miscommunications.I can relate to my friends when they say they are â€Å"having dibs† on or going â€Å"bonkers† over some girls they see in the campus or on the street, or when they say that we need to â€Å"pull an all-nighter† before the day of the examinations, and such.Learning words or phrases that are commonly used in this country has made it easier for me to understand what other people mean, making it also easy to respond, make judgments, state opinions, and such. Moreover, it has boosted my self-confidence in opening conversations with other people creating more opportunities for me to learn the English language effectively.

Analysis of West Side Story

Western stories The story of the West was published in 1961 as a melodrama performance in New York. She has the same theme as Shake Spear, Romeo, Juliet. Because it is about two lovers and that relationship can not be accepted by others because of a background conflict. The artistic meaning of this movie can be seen everywhere in the movie. It seems unrealistic to pretend to pick up and mix during the fight, but all dances, songs, performances, and pretend fights are thorough and very good. To discuss the historical development of musicals, this report analyzes Leonard Bernstein 's musical West Side Story. That is how the screen version of 1961 adapts to the revival stage of 2009 Broadway to accommodate contemporary audiences and by using ideas and styles to influence the social and economic impact of the work Indicates whether to reflect their time. The confrontation between the two hostile groups, which is very important to the story, could be further interpreted as a conflict betw een the Catholic and the Jewish community, which was the first intention of Bernstein. But Bernstein grasped the idea that New York's current ethnic tension caused by immigrants in Puerto Rico will provide a stronger story, and he gave him a series of Latin American dance rhythms I will see the opportunity to use without a doubt. He said later ...: West Side Story is recording by Arthur Laurents, music by Leonard Bernstein, lyrics by Stephen Sondheim, and choreography by Jerome Robbins. This is said to be an interpretation of Shakespeare's Romeo and Juliet. The story of West End and Romeo and Juliet have many similarities and differences in many respects. Work Romeo and Jurriette began in Verona in 1594. In contrast, the story of the Western Region was held in New York City in 1957. Most of the characters in the western district can be seen as modern versions of many characters in the Shakespearean plays. Romeo and Juliet. Tony can be regarded as Romeo, Maria as Juliet, Jet as Monta ge, Shark as Nurse, Leave as Mercurio, Bernardo as Tilbert, Dr. Flare Lawrence, Baltazar. Chino plays Paris, the police acts the prince, Anybodys and Baby John play Bemborio. The story of West End and many scenes of Romeo and Juliet are very similar. West Side Story: The West Side Story is an example between texts and talked about Shakespeare's tragedy Romeo and Juliet contemporaneously. Tony (Romeo) and Maria (Juliet) are in love. But hostile groups whose families and their loyalty depend on are prohibited from their love. The story of West End creates a new original story using Romeo and Juliet's theme (such as the end of taboo love and tragedy).